HARNESSING ALKALINE-PH REGULATABLE PROMOTERS FOR EFFICIENT METHANOL-FREE EXPRESSION OF ENZYMES OF INDUSTRIAL INTEREST IN KOMAGATAELLA PHAFFII

Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii

Abstract Background The yeast Komagataella phaffii has become a very popular host Toddler Sunglasses for heterologous protein expression, very often based on the use of the AOX1 promoter, which becomes activated when cells are grown with methanol as a carbon source.However, the use of methanol in industrial settings is not devoid of problems, and t

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PEDOT:PSS-Based Piezo-Resistive Sensors Applied to Reinforcement Glass Fibres for in Situ Measurement during the Composite Material Weaving Process

The quality of fibrous reinforcements used in composite materials can be monitored during the weaving process.Fibrous sensors previously developed in our laboratory, based on PEDOT:PSS, have been adapted so as to directly measure the mechanical stress on fabrics under static or dynamic conditions.The objective of our Mounts research has been to dev

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Comprehensive Analysis of the Transcriptome-Wide m6A Methylation in Mouse Pachytene Spermatocytes and Round Spermatids

Spermatogenesis, an efficient and complex system in male germline development, requires a series of elaborately regulated genetic events in which diploid spermatogonia differentiate into haploid spermatozoa.N6-methyladenosine (m6A) is an important epigenetic RNA modification that occurs during spermatogenesis.ALKBH5 is an m6A eraser and knocking ou

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