Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii
Abstract Background The yeast Komagataella phaffii has become a very popular host Toddler Sunglasses for heterologous protein expression, very often based on the use of the AOX1 promoter, which becomes activated when cells are grown with methanol as a carbon source.However, the use of methanol in industrial settings is not devoid of problems, and t